Extended Data Fig. 5: LPCAT1 increases the degree of saturation of membrane PL.
From: LPCAT1-mediated membrane phospholipid remodelling promotes ferroptosis evasion and tumour growth
a, Representative images of membrane azide-fluor 488 and integrin β1 fluorescence intensity in the indicated cells treated with ALA-alkyne (5 μM, 10 h) or OA-alkyne (5 μM, 10 h) normalized against integrin β1 fluorescence intensity. Scale bar, 10 μm or 2 μm. b-c, Quantification of membrane azide-fluor 488 fluorescence intensity in the indicated cells treated with PA-alkyne (5 μM, 10 h), or OA-alkyne (5 μM, 10 h), or ALA-alkyne (5 μM, 10 h), or AA-alkyne (5 μM, 10 h) normalized against integrin β1 fluorescence intensity. d-f, Relative membrane PA-488 intensity was examined in RSL3 or erastin-treated cells at the indicated times normalized against integrin β1 fluorescence intensity. Data are presented as mean ± SD, n = 6 biologically independent samples in a-f. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test in b-c. NS, not significant.
