Extended Data Fig. 2: Characteristics of ERK signal dynamics and expression levels of Kras and Hras.
a, Representative two-photon time lapse frames of the wild type late growth hair follicle expressing the ERK biosensor showing wave-like ERK signal propagation in the ORS. In this example, the wave initiated from the middle ORS and propagated towards both the upper and lower ORS. Scale bar, 20 µm. b, Back skin cells at Anagen were processed for flow cytometry and gated for ORS cells using Lgr5-GFP and K14-H2BmCherry. Sorted ORS cells were then used to conduct qRT-PCR to compare the expression levels between Kras and Hras. n = 4 mice. ns, not significant, p = 0.0852. Two-sided paired t-test was used to calculate p value. Scale bar, 20 µm. c, Pulsing frequency of the ERK signal in the upper and lower ORS cells of the control and KrasG12D hair follicles. n = 236 upper and 162 lower ORS cells in 3 wild type mice, 1666 upper and 91 lower ORS cells in KrasG12D mice. ns, not significant, p = 0.6385 and 0.0571. Two-sided unpaired t-test was used to calculate p value. d, Cumulative ERK activity of the upper and lower ORS cells of the control and KrasG12D hair follicles. The same cells in c were analysed. ns, not significant, p = 0.5064, **, p = 0.0058. Two-sided unpaired t-test was used to calculate p value. Data are presented as mean with individual data points in c and violin plots with median and quartiles in d. Scale bar, 20 µm.
