Extended Data Fig. 8: Analyses of enhancer activities in oocytes.
a, Representative images of H3K27ac immunostaining (green) and EU staining (red) in DMSO or plumbagin-treated FGO-NSN and FGO-SN. DNA was stained with DAPI (blue). Scale bar, 20μm. b, Bar charts showing the relative intensities of H3K27ac or EU in DMSO (blue) or plumbagin (red) treated FGO-NSN and FGO-SN. The dots indicate the relative intensities of individual oocytes. The numbers of oocytes used: 7, 13, 7, and 5 for H3K27ac (top), and 11, 17, 8, and 9 for EU staining (bottom). P-values (t-test, two-sided) are also provided. c, Line charts showing H3K4me3 signals at CAGE-enhancers and H3K4me3-marked distal H3K27ac peaks in Mll2 control and KO oocytes40. Pie charts showing the percentages of CAGE-enhancers and H3K4me3 at distal H3K27ac peaks in Mll2 KO oocytes compared with wild-type. d. The UCSC browser views showing CAGE in GO-P14 (ref. 46), H3K27ac, and H3K4me3 in FGOs. Putative enhancer regions defined by CAGE (red arrows) or distal H3K27ac (blue shades) are indicated. Source numerical data and unprocessed blots are available in source data.
