Extended Data Fig. 10: TCF3/12 regulate oocyte transcription and development.

a. Heatmaps showing STARR-seq signals (RNA/DNA input) and TCF3/12 motif densities (counts per kb) for enhancer candidates (including those showing positive and negative STARR-seq signals) and negative controls. b. Bar charts showing TCF3/12 motif enrichment (-log10 p-value, hypergeometric test with Bonferroni correction, one-sided from HOMER⁵⁷, Methods) in enhancers previously evaluated by STARR-seq and negative controls. Ranks of TCF3/12 motifs among all motifs are also shown. c, qPCR results showing relative RNA levels of Tcf3 and Tcf12 in wild-type and Tcf3 or Tcf12 mKO GO-P5 oocytes (n=3 biological replicates). P-value (t-test, two-sided) is also shown. d, qPCR results showing relative RNA levels of Tcf3 and Tcf12 in WT and Tcf3/12 DKO GO-P5 oocytes (n=3 biological replicates). P-value (t-test, two-sided) is also shown. e, Western blot showing protein levels of TCF3 and TCF12 in WT and Tcf3/12 DKO GO-P5 ovaries (n=3 biological replicates). f, Immunohistochemistry (IHC) showing TCF3 and TCF12 in WT (n=5) and Tcf3/12 DKO (n=5) ovaries. Scale bar, 50 μm. g, RNA expression and ribosome-protected fragment (RPF, indicating translation level) levels of Sp1 from FGOs to late two-cell embryos are shown⁹⁸. Source numerical data and unprocessed blots are available in source data.