Extended Data Fig. 1: Validation of H3K27ac ChIP-seq data in mouse oocytes and early embryos. | Nature Cell Biology

Extended Data Fig. 1: Validation of H3K27ac ChIP-seq data in mouse oocytes and early embryos.

From: Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators

Extended Data Fig. 1

a, Top, immunostaining showing H3K27ac signals in GO-P7 (n=9), GO-P10 (n=8), FGO-NSN (non-surrounded nucleolus) (n=10), FGO-SN (surrounded nucleolus) (n=4), MII oocyte (n=8), one-cell PN5 (n=16), early two-cell (n=19), late two-cell (n=9), 8-cell embryos (n=6), and blastocyst (n=5). One representative image from 3 independent experiments is shown. Scale bar, 20 μm. Bottom, boxplot showing the ratio of H3K27ac to DAPI intensity in immunostaining. The median is indicated by the center line. The bottom, top edges, and whiskers represent the 10th and 90th percentiles and 1.5 times the interquartile range (IQR), respectively. b, The UCSC browser view showing H3K27ac signals at each stage with two biological replicates. c, Heatmap showing the pairwise Pearson correlation between each sample for their H3K27ac levels in oocytes, early embryos, and tissues. Source numerical data and unprocessed blots are available in source data.

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