Extended Data Fig. 6: Proteomics analysis of VARS depleted cells reveals an enrichment in valine amino acid.
From: Valine aminoacyl-tRNA synthetase promotes therapy resistance in melanoma
a, Volcano plots showing p-values (−log10) versus log2 fold change of proteomics analysis of melanoma M395 RES depleted for VARS as compared to the control (two-tailed unpaired t-test of n = 3 independent replicates), Blue: up-regulated; red: down-regulated proteins. b, Heatmap representing amino acids analysis of proteins upregulated in M395 RES depleted for VARS as compared to control. Chi-squared test was performed. Colour bar, fold change. c, Volcano plot listing detected aminoacyl-tRNA synthetases in proteomics analysis of M395 RES cells upon VARS depletion as compared to control (two-tailed unpaired t-test of n = 3 independent replicates). d, Volcano plots showing p-values (−log10) versus log2 fold change of transcriptomic analysis of melanoma M395 RES depleted for VARS as compared to the control; Black: up-regulated; Orange: down-regulated proteins (two-tailed unpaired t-test of n = 3 independent replicates). e, f, Volcano plots of ribosome (e) and polysome (f) sequencing highlighting the five identified candidates (HADH, KIF13B, SLC7A5, QDPR and GOLT1B) (two-tailed unpaired t-test of n = 3 independent replicates).
