Fig. 5: VARS regulates the translation of valine rich transcripts.
From: Valine aminoacyl-tRNA synthetase promotes therapy resistance in melanoma
a,b, OP-puro (a) and HPG (b) incorporation relative to control in M395 RES melanoma cultures depleted or not depleted of VARS (fold mean ± s.e.m. of n = 3 independent replicates; a two-tailed unpaired t-test was performed). c, Metagene density profiles depicting global RPFs in coding sequences (CDS) in M395 RES cells after VARS depletion (green) as compared with control (blue). The y axis shows the average intra-gene normalized density of RPFs for each transcript. UTR, untranslated region. d, Diricore analysis plots at the E site, P site and A site (positions 9, 12 and 15, respectively), analysing differential RPFs density at codons in M395 RES shVARS as compared with shCTRL. e,f, Violin plots representing the log2 FC of significantly regulated transcripts of shVARS as compared to control in ribosome sequencing (e) or polysome sequencing (f). The transcripts are sorted based on their enrichment (top 25%) or impoverishment (bottom 25%) in the indicated amino acids (two-tailed unpaired t-test).
