Extended Data Fig. 7: TMED10-positive ERGIC compartment is required for UcPS.
From: A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion
a, SIM analysis and 3D view of GFP-ERGIC53 and TMED10-V5 in HeLa cells. Scale bar, 5 μm in overview and 2 μm in the zoomed in. b, SIM analysis of colocalization of TMED10 regions separated from ERGIC53 with GM130, SEC16, COPB2, SEC31, Rab5A, Rab7A or Rab2A. Scale bar, 5 μm in overview and 1 μm in the zoomed in. c, SIM analysis of colocalization of ssGFP with ERGIC53 or TMED10. Scale bar, 5 μm in overview and 2 μm in the zoomed in. Diagram showing the percentage of ssGFP colocalized with ERGIC53 or TMED10 per cell. P values are indicated (two-tailed t test, n=51 cells). d,e, IL-36α (d)/IL-33 (e) secretion in HEK293T cells using RUSH system. Diagram showing the quantification of secretion (mean ± SD). P values are indicated (two-tailed t test, n=3 experiments). *, Non-specific band. L, low exposure; H, high exposure. f–h, ssGFP (f)/IL-6 (g)/IL-10 (h) secretion in HEK293T cells using RUSH system. Diagram showing the quantification of secretion (mean ± SD). P values are indicated (two-tailed t test, n=3 experiments, ns, no significant difference). *, Non-specific band. i, SIM analysis of colocalization of GM130 with ERGIC53 or TMED10. Scale bar, 5 μm in overview and 2 μm in the zoomed in. Diagram showing the percentage of GM130 colocalized with ERGIC53 or TMED10 per cell. P values are indicated (two-tailed t test, n=52 cells). Exact P values are presented in Source numerical data. Source numerical data and unprocessed blots are available in Source data.
