Extended Data Fig. 2: Rab1A, Rab1B and Rab2A regulate the secretion of a series of UcPS cargoes.
From: A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion
a–c, ssGFP secretion in HEK293T cells co-transfected with ssGFP and BFP/BFP-Rab1A (a)/1B (b)/2A (c) plasmids with or without BFA treatment (0.5 ng/μl). *, Non-specific band. d,e, IL-6 (d)/IL-10 (e) secretion in HEK293T cells transfected with control siRNA (si Ctr) or siRNAs against Rab1A/1B/2A. f–h, ssGFP (f)/IL-6 (g)/IL-10 (h) secretion in HEK293T cells transfected with control siRNA (si Ctr) or siRNAs against Rab1A and Rab1B. i–k, Secretion of IL-18 in HEK293T cells co-transfected with IL-18-FLAG and GFP/GFP-Rab1A (i)/1B (j)/2A (k) plasmids. l–n, Secretion of IL-33 in HEK293T cells co-transfected with IL-33-FLAG and GFP/GFP-Rab1A (l)/1B (m)/2A (n) plasmids. o–q, Secretion of IL-36α in HEK293T cells co-transfected with IL-36α-FLAG and GFP/GFP-Rab1A (o)/1B (p)/2A (q) plasmids. *, Degradation band. r–t, Secretion of HSPB5-FLAG in HEK293T cells co-transfected with HSPB5-FLAG and GFP/GFP-Rab1A (r)/1B (s)/2A (t) plasmids. u–w, Secretion of Galectin-3 in HEK293T cells co-transfected with Galectin-3-FLAG and GFP/GFP-Rab1A (u)/1B (v)/2A (w) plasmids. a–w, The data are representatives of three independent experiments. R1A, Rab1A; R1B, Rab1B; R2A, Rab2A. Source unprocessed blots are available in source data.
