Extended Data Fig. 5: Translation regulation of suntag-nanos is mediated by nanos 3′UTR.
From: Direct observation of translational activation by a ribonucleoprotein granule
a, Schematics of transgenic constructs of UAS-suntag-nanos, UAS-suntag-nanos-SREmut, and UAS-suntag-nanos-tubulin3′UTR. The red asterisks in nanos 3′UTR represent the two SREs mutated in the construct. b, Representative images of embryos expressing suntag-nanos (top), suntag-nanos-SREmut (middle), and suntag-nanos-tubulin3′UTR (bottom). Note that the translation activities in the soma of embryos expressing suntag-nanos-SREmut and suntag-nanos-tubulin3′UTR are higher than suntag-nanos. Blue, Vasa; magenta, suntag smFISH; green, anti-GCN4.
