Extended Data Fig. 2: Quantitative detection of SunTag with anti-GCN4 versus scFv-GFP.
From: Direct observation of translational activation by a ribonucleoprotein granule
a, Schematics of stage-1, stage-3, and stage-5 embryos. Germplasm or pole cells are labeled in blue. Outlined regions are imaged and presented in panel (B). b, Example images of stage-1, stage-3, and stage-5 embryos expressing suntag-nanos. SunTag is stained by anti-GCN4 (green); suntag mRNA is stained by smFISH (magenta); germplasm or pole cells are marked by Vasa-mApple (blue). c, Images of a stage-1 embryo expressing suntag-nanos stained by anti-GCN4 (top) and a stage-1 embryo from a w1118 female stained by anti-Nanos (bottom). d, Images of germplasm in stage-1 embryos (top) and pole cells in stage-5 embryos expressing suntag-nanos. SunTag (green) is stained by anti-GCN4 (left) or by endogenous scFv-GFP (right); suntag mRNA is stained by smFISH (magenta). e, Quantification of the percentage of mRNA foci co-localized with SunTag staining signal in stage-1 germplasm and stage-5 pole cells when SunTag is stained by scFv-GFP or anti-GCN4. nst1-scFv = 6, nst1-GCN4 = 7, nst5-scFv = 5, nst5-GCN4 = 4; n refers to the number of embryos. Data are the mean ± s.d.
