Fig. 1: Chromosome fusions induce spindle defects from 1n = 4.
a, Chromosome lengths of wild-type (16 chr) and 3 chr S. cerevisiae. Vertical lines indicate positions of centromeres. b, Maximum growth rates of fused-chromosome strains on synthetic complete medium with 2% dextrose (SCD). Boxes show the means and s.d. Means were compared with the wild type using a two-tailed Student’s t-test (from left to right, n = 8, 8, 8, 8, 8, 7, 8 and 8 independent population measurements). c, Montage of SPB (Spc42-mCherry) dynamics during mitosis for 16- and 3-chromosome strains. Scale bar, 2 µm; intervals are 3 min. The time point with maximal SPB separation during anaphase was set to zero. Open arrows indicate SPB doubling. d, Distance between SPBs over time. For normalization, the time point with maximal SPB separation during anaphase was set to zero. The vertical line represents the inflection point (~start of anaphase). e, The time from SPB doubling to max. anaphase separation. Boxes represent the mean and s.d. Means were compared using a two-tailed Student’s t-test (from left to right, n = 18, 18, 20, 39 and 34 independent single-cell measurements). chr, chromosome. Source numerical data are available in source data.
