Extended Data Fig. 5: SAC activation in fused-chromosome strains.
(a) Epistatic effect of MAD2 deletion. Boxes represent means and standard deviation. (b) Maximum growth rates of fused-chromosome strains with and without RAD9 deletion. Boxes represent means and standard deviations. Means were compared using a two-tailed Student’s t-test (from left to right, n = 16, 16, 16, 16; independent population measurements). (c) Maximum growth rates of fused-chromosome strains with and without 10 mM hydroxyurea (HU). Boxes represent means and standard deviations. Means were compared using a two-tailed Student’s t-test (from left to right, n = 13, 16, 16, 15; independent population measurements). (d) Maximum growth rates of fused-chromosome strains with and without BUB2 deletion. Boxes represent means and standard deviations. Means were compared using a two-tailed Student’s t-test (from left to right, n = 19, 21, 21, 21; independent population measurements). (e) Distance between SPBs over time in MAD2∆ cells. For normalization, the time point with maximal SPB separation during anaphase was set to zero. (f) Western blot analysis of Pds1 levels after G1 release for 16- and 3-chromosome strains, focused on the second cell cycle after release. Cells were collected at the indicated time points. Ponceau S staining was used as a loading control. Pds1-normalized values are shown in the bar plot at the bottom. An independent repeat of this experiment with analysis of the first cell cycle can be found in Fig. 5d. Source numerical data and unprocessed blots are available in source data.
