Extended Data Fig. 1: Growth and mitotic defects in fused-chromosome strains.
(a) Maximum growth rates of fused-chromosome strains on synthetic complete medium with 2% dextrose (SCD). Boxes show the means and standard deviation. Means were compared using a two-tailed Student’s t-test (from left to right, n = 8, 7, 7, 8, 8, 8, 7, 5, 7, 5; independent population measurements). (b) Distance between SPBs over time for different fusion strains. For normalization, the time point with maximal SPB separation during anaphase was set to zero. (c) Expanded cells with spindle defects. Cells were labelled with pan protein label NHS ester and for tubulin. Scale bar = 10 µm, expansion factor = 4.18. (d) Montage of spindle dynamics over time (CloverGFP-tub1). Scale bar = 5 µm, intervals are 1 min. Closed arrows point to an example of increased spindle curvature, open arrows to an example of the whole spindle moving into the daughter cell. (e) Spindle curvature (%), calculated as the total spindle length relative to the distance between spindle pole bodies (SPBs). Data are represented as violin plots with boxes representing the interquartile range, the lines representing the median and the whiskers indicating the minimum and maximum values. Black dots represent outliers. n = 50 for each genotype; independent single-cell measurements. Distributions were compared using Kolmogorov-Smirnov tests. (f) Montage of nuclear envelope (Hmg1-mCherry) dynamics over time. Scale bar = 5 µm, intervals are 1 min. Source numerical data are available in source data.
