Extended Data Fig. 1: Transgenerational self-sterility of mstr mutants is caused by germline feminization.
From: Noncanonical inheritance of phenotypic information by protein amyloids
(a) Schematic of germ cell sex differentiation in WT hermaphrodites and sterile mstr worms. L4, 4th larval stage. YA, young adult. Ad, adult. d, distal. p, proximal. (b) Penetrance of sterility in worms of the indicated genotype at the indicated generation and maintenance temperature. (c) Representative DIC micrographs of WT and sterile mstr-2 late generation worms. Dashed line, germline. Bar, 20 μm. (d) Fluorescence and DIC micrographs of WT and mstr hermaphrodites showing expression of the oocyte marker RME-2::GFP. Inset, most proximal germ cells. Dashed line, germline. Bars, 20 μm. (e) Fluorescence and DIC micrographs of somatically male mstr worms showing expression of the oocyte marker RME-2::GFP. White dashed line, germline. Green dashed line, intestine Bar, 20 μm. (f) Transcriptomes of WT worms compared to mstr-1 worms (20 °C). Left panel P values, negative binomial exact test (edgeR). Right panel, ****P < 0.0001 two-tailed Mann–Whitney test. n = 3 populations of WT or mstr worms sequenced at L4 stage. Numerical data and statistical values are available in source data.
