Extended Data Fig. 2: DRP1 is required for the mechano-regulation of SREBP1-2.
a, Expression levels (CPM, counts per million) of selected SREBP1-2 target genes from RNA sequencing of D2.0R cells, selected among the genes downregulated on a soft ECM upon DRP1 shRNA (n = 3 in a single experiment). b, SREBP2 localization in MCF10A-RAS cells deprived of serum and of exogenous lipids. DRP1 was inhibited with Drpitor1a (DRP1A) (n = 72 cells (SERUM) n = 120 (NO SERUM) and n = 131 (NO SERUM DRP1A) from three experiments). c, Excitation ratio of the ROCKAR sensor in MCF10A-RAS cells cultured as in (b) (n = 47 (FULL SERUM), n = 47 (NO SERUM), n = 47 (REFEED) from two experiments). d,e, SREBP2 localization in MCF10A-RAS cells treated with the U18666 NPC1 lysosomal cholesterol transporter inhibitor (d), or depleted of COPI27 (e) (d, n = 91 (ethanol), n = 103 (U18666A), n = 99 (U18666A DRP1A); e, n = 81 (siCO), n = 76 (siCO YM), n = 89 (siCO YM DRP1A), n = 98 (siCOPIa), n = 72 (siCOPIa DRP1A), n = 63 (siCOPIb), n = 81 (siCOPIb DRP1A) from three experiments). Scale bar 10 μm. Data are presented as mean values and single points (a,c) or mean ± s.d. In b,d,e, p-values were calculated based on two-tailed Student’s t-tests on nuclear SREBP2. Source numerical data are available in the source data and Supplementary Table 1.
