Extended Data Fig. 6: Actomyosin tension inhibits fission by promoting MIEF1 phosphorylation.
a, Mitochondrial length analysis in MCF10A-RAS cells reconstituted as in Fig. 7i, but with an independent set of MIEF1-2 siRNAs (siMIEF1/2b) (siCO DMSO and siCO YM as in Fig. 7i, n = 49 (siMIEF1/2b DMSO), n = 44 (siMIEF1/2b YM), n = 45 (siMIEF1/2b WT low level YM), n = 39 (WT medium level), n = 43 (WT high level), n = 41 (SA low level), n = 39 (SA medium level), n = 44 (SA high level), n = 51 (SE low level), n = 39 (SE medium level) and n = 42 (SE high level) from three experiments). b, Immunoblotting of HA-MIEF1 isoforms reconstituted in MCF10A-RAS cells as in Fig. 7i. Consistent results were obtained in n = 2 experiments. Data are presented as mean values ± s.d. In a, p-values were calculated on punctate mitochondria based on two-tailed Student’s t-tests. Source numerical data and unprocessed blots are available in the source data.
