Extended Data Fig. 6: SE regulates the phase behaviors of MTC in plants.
From: SERRATE drives phase separation behaviours to regulate m6A modification and miRNA biogenesis
a – d, Confocal images (a) and FRAP assays (b – d) revealed that SE and MTC have similar phase behaviors when transiently expressed via (a, b, upper panels, and c) 35S and (a, b, bottom panels, and d) their native promoters in N. benthamiana. Data are mean ± s.d. of at least eight independent experiments. e, FRAP assays showed that SE and MTB condensates undergo phase transition from liquid-like to a gel- or solid- like behavior upon 1,6-HD treatment that blocks the signal recover after bleaching. Regions of interest were labelled with white circles. f, g, Confocal images (f) and statistical analysis (g) revealed the decreased MTB signal in se-1 vs Col-0. In (g), counting mode was used to analyse detectable photon for normalization. Data are mean ± s.d. of sixteen independent experiments. P value is < 0.0001, an unpaired two-sided t-test. h, Immunoblots with three-week-old plants detected the proteins in indicated fractions extracted from se-2 and se-3 vs Col-0. i, RNA-seq54 analysis exhibited that increased expression of MTB in ten-day-old seedling in se-2 vs Col-0. Data are mean ± s.d. of three independent replicates. P value is 0.011, an unpaired two-sided t-test. j, Two biological replicates of immunoblots with ten-day-old seedling detected a decreased ratio of soluble (supernatant) MTB in se-2 vs Col-0 where the amount was arbitrarily assigned a value of 1. Scale bars, 2.5 μm (a, b, e, andf). At least eight (a, b, ande), sixteen (f), and three (h, j) independent experiments were performed, and representative images are shown.
