Extended Data Fig. 5: Features of type R vessels.
From: Specialized post-arterial capillaries facilitate adult bone remodelling
a, b. High-resolution confocal images showing VEGFR3 (yellow) and VEGFR2 (red) immunostaining together with DAPI (blue). VEGFR3− VEGFR2+ capillaries (white arrowheads) around trabecular bone (TB) and nearby VEGFR3+ VEGFR2+ vessels (yellow arrowheads) in samples from young (a) and aged patients (b) are indicated. Scale bars, 200μm. c. Tile scan confocal images of EMCN+ VEGFR3− ECs in 12-week-old female and male murine femur. Trabecular bone (TB) is indicated. Scale bars, 500μm. d. Quantitation of EMCN+ VEGFR3− vessel density in 12-week-old female and male femur (n =3 mice per group). Mean ± SEM. P values, unpaired two-tailed t-test. e. EMCN (red) and VEGFR3 (blue) immunostaining showing the presence of VEGFR3− EMCN+ vessels (white arrowheads) around trabecular bone (TB) in female and male femur. Scale bars, 100μm.
