Extended Data Fig. 9: Modulation of type R vessels by anti-osteoporosis treatments.
From: Specialized post-arterial capillaries facilitate adult bone remodelling
a, Scheme for the treatment of adult mice with parathyroid hormone (PTH) or Alendronate. Black arrows indicate onset of treatment, red arrows time points of imaging. b, Confocal images of OSTEOPONTIN (blue) immunostaining of the metaphysis near the growth plate (GP) together with EMCN (red), CAV1 (green) and DAPI (white) to show the response to treatments. Scale bars, 100μm. c, UMAP visualization of sorted bone ECs from control and treatment groups with colour-coded subclusters. d, Bar plots showing the proportions of EC subpopulations in 12-week-old mice. Differences in percentage of rECs and aECs compared to control are indicated. Panels c and d show the individual samples from an integrated scRNA-seq dataset of three conditions. e, f, Confocal images showing EMCN+ VEGFR3− CAV1+ rECs (white arrowheads) around trabecular bone (TB) (e) and association of ATP6V1B1/B2+ (green) osteoclasts with EMCN+ VEGFR3− type R capillaries (f) in 12-week-old control and indicated treatment conditions. Scale bars, 50μm. g, Effect of treatments on EMCN+ VEGFR3− rECs (white arrowheads) and RUNX2+ (green) osteoprogenitors around trabecular bone (TB). Scale bars, 100μm.
