Fig. 2: Identification of remodelling endothelial cells.
From: Specialized post-arterial capillaries facilitate adult bone remodelling
a, UMAP plots showing colour-coded subclusters in juvenile and adult bone ECs. Dashed black line encompasses rEC clusters. Red arrowhead indicates expansion of type R ECs in adult. b, Bar plots showing colour-coded subclusters in juvenile and adult bone ECs. Relative representation (in %) of rECs and aECs is indicated. c, High-magnification images of femurs immunostained for EMCN (red), VEGFR3 (blue) and CAV1 (green) showing the emergence of EMCN+VEGFR3−CAV1+ (yellow) rECs (white arrowheads) around trabecular bone (TB). d, Two-photon microscopic image of immunostained EMCN+VEGFR3− rECs (white arrowheads) around TB visualized by second-harmonic generation. e, Representative confocal images of 3-, 6-, 8- and 12-week-old femurs immunostained for EMCN (red) and VEGFR3 (green). White arrowheads indicate increasing age-dependent abundance of EMCN+VEGFR3− rECs around TB. f, Quantitation of rECs (EMCN+VEGFR3− area) showing the age-dependent increase in the 3-, 6-, 8- and 12-week-old TB relative to samples from 3 weeks. n = 3 mice per group. Mean ± s.e.m. P values, one-way analysis of variance (ANOVA). g, Schematic representation of type R capillary expansion in postnatal and adult long bone. Data in a show individual samples from different age groups, whereas b is based on integrated scRNA-seq data.
