Fig. 4: Fate mapping of Aplnr+ ECs.
From: Specialized post-arterial capillaries facilitate adult bone remodelling
a, UMAP plot (derived from integrated scRNA-seq data of all age groups) illustrating Aplnr expression in rECs (arrow) but not aECs (arrowhead). Scheme of genetic fate-mapping strategy with Aplnr-CreERT2 mice. 4-OHT administration (1 mg per mouse) is indicated by black arrows and red arrows mark time points of analysis. b,c, High-resolution confocal images and tile-scan overview images of fate-tracked Aplnr-CreERT2 R26-mTmG (GFP, green) ECs in femur at 3 weeks (72 h after 4-OHT administration and Cre activation), 6 weeks (3 weeks after Cre activation) (b) and at 12 weeks (9 weeks after Cre activation) (c). Small panels show higher magnifications of TB and diaphyseal area. White arrowheads mark GFP+EMCN+ bmECs, yellow arrowheads GFP+CAV1+EMCN+ rECs, green arrowheads GFP−CAV1+ aECs and red arrowheads fate-tracked GFP+CAV1+EMCN− ECs inside arteries. White dashed lines in b and c indicate arteries and arterioles. d, Bar plots showing the proportion of area covered by CAV1+GFP+ rECs and aECs, CAV1+GFP− aECs and CAV1−GFP+ ECs at P21, 6 weeks and 12 weeks post-induction. e, Schematic illustration of genetic fate mapping of rECs in Aplnr-CreERT2 R26-mTmG femur.
