Extended Data Fig. 1: Molecular heterogeneity of endothelial cells in long bone.
From: Specialized post-arterial capillaries facilitate adult bone remodelling
a. Representative FACS plots showing gating strategies for the isolation of ECs from Cdh5-mTnG reporter bone. b. Tile scan confocal images showing sections of 6, 22 or 55-week-old Cdh5-mTnG reporter bone co-stained for EMCN (red) and VEGFR3 (blue). The avascular growth plate (GP) and regions containing bmECs, mpECs and rECs are indicated. Small panels show higher magnifications of these areas. Arrowheads mark type R capillaries. Scale bars, 100μm (overview images) and 500μm (small panels). c. Heatmap showing the expression of selected marker genes for each EC subcluster (based on integrated scRNA-seq data for all age groups). d. Representative confocal images showing immunostaining for EMCN (red) and SOX11 (green, white arrowheads), marking pECs in the young but not adult metaphysis. e. CXADR (green) staining of bmECs in young, adult, and aging femur. Growth plate (GP) and epiphysis are indicated. f. MAdCAM1 (green, white arrowheads) expression in diaphyseal bmECs in young and adult femur. Scale bars, 50μm (d), 500μm (e), 100μm (f).
