Extended Data Fig. 7: GABPA binding dynamics and comparisons with other pluripotency regulators.
From: The transcription factor GABPA is a master regulator of naive pluripotency
(a) Expression dynamics of Gabpa, Nr5a2, Tfap2c and Sox2 during mouse pre-implantation development. (b) Correlation of replicates for GABPA CUT&RUN in 8-cell embryos (left) and E3.5 ICM (right). Rp: Pearson correlation. (c) Genomic distribution of GABPA binding peaks in 8-cell embryos (left) and E3.5 ICM (right). (d) Genome browser view showing examples of the binding profiles of GABPA in 2-cell, 8-cell, E3.5 ICM, E4.5 ICM and ESCs. (e) Comparison of the GABPA direct target genes between 2-cell embryos and E4.5 ICM. (f) Examples of genome browser view illustrating the GABPA binding (shaded) dynamics during preimplantation development. (g) Heatmap comparison of GABPA and TFAP2C binding profiles at promoter, intron and distal regions in 2-cell, 8-cell and E3.5 ICM. (h) Comparisons of GABPA (E4.5 ICM) and TFAP2C (E3.5 ICM) affected ICM genes (left) and EPI genes (right). (i) Comparisons of TFAP2C affected ICM genes (left) and EPI genes (right) in 8-cell and E3.5 ICM. (j) Comparisons of GABPA (E4.5 ICM) and SOX2 (E3.5 ICM) affected ICM genes (left) and EPI genes (right). (k) Comparisons of GABPA and SOX2 affected ICM genes (left) and EPI genes (right) in ESCs. GABPA CUT&RUN and RNA-seq in e–g showed the pooled results from two biological replicates.
