Fig. 4: GABPA regulates EPI genes in E4.5 ICM by both promoter and distal binding.

a, The genomic distribution of GABPA binding peaks in E4.5 ICM based on CUT&RUN data. b, Heatmaps showing the DEGs at E4.5 ICM after dTAG13 treatment, as well as the GABPA binding and several TF motif occurrences around the TSS of the indicated gene groups. c, The percentage of the downregulated early and late ICM genes (left), and downregulated ICM genes with GABPA promoter binding (right) at E4.5 ICM. d, Examples of early ICM genes with GABPA promoter binding (shaded) at E4.5 ICM. e, Cumulative distributions of the distance from TSS to the nearest distal GABPA peaks for each group of genes at E4.5 ICM. f, Heatmaps showing all the GABPA peaks at promoters, introns and distal regions in E4.5 ICM, and ATAC–seq and H3K27ac signals at these GABPA peak regions with or without dTAG13 treatment. C: peak centre. g, An example of the genome browser view of GABPA binding (shaded), ATAC and H3K27ac signals in E4.5 ICM with or without dTAG13 treatment. All RNA-seq, GABPA CUT&RUN, ATAC–seq and H3K27ac CUT&RUN analysis were performed using two independent biological replicates. The pooled results for ATAC–seq and H3K27ac CUT&RUN are shown in f and g.