Fig. 6: GABPA genomic binding dynamics during pluripotency establishment and its relationship with other pluripotency regulators.

a, Heatmaps showing the dynamics of GABPA genomic binding profile from two-cell (2C), eight-cell (8C) and E3.5 ICM to E4.5 ICM at promoter, intron and distal regions. C: peak centre. b, A comparison of GABPA and NR5A2 binding profiles and motif occurrence at promoter, intron and distal regions at two-cell and eight-cell stages. c, GABPA and TFAP2C binding profiles at promoters of early and late ICM genes in two-cell, eight-cell and E3.5 ICM. d, The genome browser view of two examples showing GABPA and TFAP2C binding dynamics. Promoter binding regions are shaded. e, GABPA and SOX2 binding profiles at promoter, intron and distal regions in E3.5 ICM and E4.5 ICM. f, GABPA and SOX2 binding profiles at promoters of early and late ICM genes in E3.5 ICM and E4.5 ICM. g, The genome browser view of two examples showing the binding (shaded) of GABPA and SOX2 in E3.5 ICM and E4.5 ICM. h, Comparisons of GABPA (E4.5 ICM) and SOX2 (ES cells) affected ICM genes (left) and EPI genes (right) after dTAG13 treatments. i, The percentage of ICM and EPI genes regulated by GABPA and SOX2 in E4.5 ICM and ES cells. j, A model illustrating that different TFs including TFAP2C, SOX2 and GABPA play major roles at different stages during pluripotency establishment (from the eight-cell to the E4.5 ICM). CUT&RUN analysis in a–g was performed using two independent biological replicates, and pooled results were used.