Extended Data Fig. 1: Generation of GabpadTAG/dTAG mice.
From: The transcription factor GABPA is a master regulator of naive pluripotency
(a) Diagram of Fkbp(F36V)-HA knock-in at the 3’ end of Gabpa gene. (b) Genotypes of pups from heterozygous GabpadTAG/+× GabpadTAG/+ crosses. (c) Confirmation of GABPA degradation with 4h-dTAG13 treatment in zygote (left) and 4-cell (middle), and anti-HA fluorescence intensity quantification (right) (n = 3). Anti-HA antibody was used for GABPA-HA fusion protein staining. DNA, Hoechst 33342. Scale bar, 20 μm. (d) Quantification of the fluorescence intensity of GABPA in Fig. 1g (n = 3). (e) Embryo development of wild-type embryos with or without dTAG 13 treatment. The developmental rate (left) and representative pictures of E3.5 embryos (middle and right). Scale bar, 50 μm. The independent experiments were repeated three times. Data in c d and e are presented as mean values ± SD. P-values were calculated with two-sided Student’s t-test.
