Extended Data Fig. 5: BTR and SLX4 promote mitotic survival following low dose IR.
From: Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage
A) Western blots of whole cell extracts from 3F HeLa. The asterisk denotes a non-specific band (representative of n = 2). B) RT-qPCR of siRNA transfected 3F HeLa (n = 3, two-tailed paired t-test t, df, and 95% CI are shown in Source Data). C, D) First mitosis outcome in siRNA transfected and G2 enriched 3F HeLa ± 2 Gy IR (mean ± SEM n = 2 for (C) and 3 for (D), two-sided Fisher’s exact test of N). E-G) Mitotic duration for the cells in Extended Data Fig. 5D (E), Extended Data Fig. 5C (F), and Fig. 3i (G) (mean ± SEM and Kruskal-Wallis uncorrected Dunn’s multiple comparisons test of N). All panels, N = individual cells across all replicates, n = biological replicates, ns = not significant. Source numerical data and unprocessed blots are available in source data.
