Extended Data Fig. 6: Targeting RAD51 or WAPL differently rescues immediate mitotic death.
From: Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage
A) Cohesion fatigue in cytogenetic chromosome spreads from 14 Gy irradiated HT1080 6TG, scale bar = 5 µm (representative of n = 3). B) First mitosis outcome in 14 Gy irradiated G1 cells from asynchronous and siRNA transfected 3F HeLa (mean ± SEM, n = 2, 3, 2, 3 left to right, two-sided Fisher’s exact test of N). C) Western blots of whole cell extracts from parental and WAPL knockout (KO) 3F HT1080 6TG (representative n = 2). D) Cohesion fatigue in parental or WAPL KO HT1080 6TG cultures ± 14 Gy IR and/or siRNA (mean ± SEM of n = 3, two-sided Fisher’s exact test of N). E) First mitosis outcome in G2 enriched and 14 Gy irradiated parental or WAPL KO 3F HT1080 6TG ± siRNA (mean ± SEM, n = 3, two-sided Fisher’s exact test of N). All panels, N = individual cells across all replicates, n = biological replicates, ns = not significant. Source numerical data and unprocessed blots are available in source data.
