Extended Data Fig. 7: Distinct apoptotic pathways underpin immediate and delayed lethality following IR.
From: Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage
A) HeLa proliferation measured through IncuCyte live imaging (mean ± SEM, n = 3). B) RT-qPCR from HeLa. Fold change presented relative to cells collected prior to IR (T0), normalized to 1 and depicted as a red dotted line (mean ± SEM, n = 3, RM one-way ANOVA with Fisher’s LSD multiple comparisons test, F and 95% CI in Source Data). C) Western blots of HeLa whole cell extracts. #1 are transduced with diluted 1:2 lentivectors and #2 with crude viral supernatant (n = 1). D, E) All cell cycle outcomes (D) and mitotic duration (E) in HeLa ± shRNA and/or 20 Gy IR (D, mean ± SEM n = 2, two-sided Fisher’s exact test of N; E, mean ± SEM and Kruskal-Wallis uncorrected Dunn’s multiple comparisons test of N). F, G) IncuCyte live imaging of a Caspase 3/7 fluorescence reporter (F) and relative apoptosis (G, area under the curve from F) in HeLa ± 14 Gy and/or 40 µM Z-IETD-FMK (mean ± SEM, n = 4; G, two-tailed paired t-test, t = 8.5, df = 3, 95% CI = -0.55 to -0.25). H, I) RT-qPCR of siRNA transfected HeLa. Fold change in gene expression relative to control siRNA immediately prior to IR (T0) and normalized to 1 (mean ± SEM n = 3; I, RM one-way ANOVA with Fisher’s LSD multiple comparisons test relative to control siRNA + 14 Gy IR, F and 95% CI in Source Data). J, K) IncuCyte live imaging of a Caspase 3/7 fluorescence reporter quantified in Fig. 7i (mean ± SEM; n in Fig. 7i legend). All panels, N = individual cells across all replicates, n = biological replicates, ns = not significant. Source numerical data and unprocessed blots are available in source data.
