Extended Data Fig. 8: Pharmacological or genetic alteration of DSB repair impacts interferon expression following IR.
From: Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage
A) RT-qPCR from HeLa ± 14 Gy IR, siRNA, and/or 0.2 µM VE-822 (mean ± SEM n = 3 except control n = 6, and WAPL siRNA n = 4, Mixed-effect one-way ANOVA with Fisher’s LSD multiple comparisons test, F and 95% CI in Source Data). B) RT-qPCR of siRNA in Fig. 8a and Extended Data Fig. 8A (mean ± SEM of n = 3). C) Model. Activity of DSB repair pathways across cell cycle (top). Interpretation of repair kinetics following DSB induction in G1, S, or G2 phase cells (below) as a function of DSB load and cell cycle progression. A red dotted line indicates a theoretical DSB threshold that separates functional (green shading) and toxic (magenta shading) HR. Source numerical data are available in source data.
