Extended Data Fig. 1: Distinct modes of cell death following lethal genomic damage in p53 compromised cells.
From: Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage
A) Examples of aberrant mitoses from irradiated 3F HeLa (representative of n = 3). Time is hrs:min relative to nuclear envelope breakdown (NEBD). Scale bar = 50 µm. B) Replicate means from Fig. 1c (mean ± SEM left to right n = 4, 3, 4, 4, 3, 4, 3, 3, 3; two-sided Fisher’s exact test of N shown in Fig. 1c). C) Interphase lethality from Fig. 1c (mean ± SEM left to right n = 3, 3, 4, 4, 3, 4, 3, 3, 3. D) Outcomes over the entire 120-hour live imaging experiment in Fig. 1g as a function of cell cycle phase at irradiation (mean ± SEM left to right of n = 4, 3, 4, 4, 3, 4, 2; two-sided Fisher’s exact test of N). E) Mitotic duration from Fig. 1g delineated by outcome and cell cycle phase at IR (mean ± SEM left to right n = 4, 3, 4, 4, 3, 4, 3). F) First mitosis outcome in 3F HT1080 6TG as a function of cell cycle phase at irradiation (mean of n = 2; two-sided Fisher’s exact test of N). G, H) Cell fate map from 3F A549 (G, n = 1) and 2 F IMR90 (H, n = 2) cultures following mock or IR treatment. Each line represents a single cell relative to IR at T0. For mock-treated cultures, only the first cycle was recorded. For irradiated cultures, cells were tracked for 120 h or until migration from the imaging frame. The asterisk depicts the only cell death event observed. All panels, N = individual cells across all replicates, n = biological replicates, ns = not significant. Source numerical data are available in source data.
