Extended Data Fig. 2: IDF-MTX complex does not dissipate the mitochondrial membrane potential.
From: Mitochondrial YME1L1 governs unoccupied protein translocase channels
(a) HeLa6-TetOn-IDF cells were treated with DMSO or Dox/MTX for 24 hours or further treated with CCCP for the last 1 hour. Mitochondrial membrane potential was visualized with TMRE staining. DIC, differential interference contrast. Bars, 50 μm. All images are representative of at least two independent experiments. (b) Quantification of TMRE signals in (a). The TMRE-positive area values per cell were shown as jittered dots. The data were obtained from two independent experiments (n=207 for DMSO, n=225 for Dox/MTX, n=206 for Dox/MTX+CCCP=206). Horizontal lines are displayed as the median. Statistical significance was assessed by one-way ANOVA with Dunnett’s multiple comparisons test (ns, not significant; *** p<0.001). Source numerical data are available in source data.
