Extended Data Fig. 5: IDF plugging induces selective degradation of TIMM17A and TIMM23.
From: Mitochondrial YME1L1 governs unoccupied protein translocase channels
(a) The cell lysates prepared as in Fig. 5b were analysed by IB with antibodies against TOM subunits and TIMM50. All blots are representative of four independent experiments. (b) Volcano plot representation of the change in the putative YME1L1 substrates upon IDF plugging. The quantitative proteomic data was the same as in Fig. 5a but with different annotations. Class I proteins are the 29 putative YME1L1 substrates that are downregulated under hypoxic conditions; Class II proteins are the remaining 35 putative YME1L1 substrates that accumulated in YME1L1 KO MEF cells during normoxia. The annotation of YME1L1 substrates was retrieved from the previous report23. Unprocessed blots are available in source data.
