Extended Data Fig. 1: Differential cell movements between epiblast and primitive endoderm contribute to fate segregation in the ICM.
From: Coupling of cell shape, matrix and tissue dynamics ensures embryonic patterning robustness
a. Representative images of an E3.5 blastocyst and corresponding isolated ICM after immunosurgery with quantification of total cell numbers in the ICM before and after immunosurgery. Paired samples t-test, two-sided, p = 0.644 with n = 24 embryos. b. Schematic representation for quantification of the sorting score in isolated ICMs. Sorting score values range from s = -1 to s = 1, with s = -1 indicating EPI enveloping PrE, s = 0 indicating salt-and-pepper distribution of cell types, and s = 1 indicating PrE enveloping EPI. c. Representative immunofluorescence images of ICMs isolated at stage E3.5 (left), compared to ICMs isolated at stage E3.5 followed by 24-hour in vitro culture (right), and quantification of the sorting score in these experimental groups. Two-sided Mann–Whitney U-test, p = 1.45e−07 and n = 20, 18 ICMs for the two groups, respectively. Scale bar, 20 μm. ns, non-significant, ***p ≤ 0.001.
