Fig. 1: Human PHB1 and PHB2 are primarily localized at the CMs.
a,b, Confocal microscopy of living cells expressing PHB1–DK (a; n = 3 independent experiments) and PHB2-DK (b; n = 3 independent experiments) at endogenous levels. Mitochondria were labelled using MitoTracker deep red FM (magenta). DK fluorescence is shown in green. c, Immunogold EM of PHB1–DK and PHB2–DK (top) and WT (bottom) cells. Representative micrographs for each decoration with the indicated antibody are shown and the localization of gold particles is indicated by boxes. d, Gold particle density in the CM and IBM (normalized to the same membrane length unit). e, FRAP analysis of PHB2–DK compared with mito-DK. The circle represents the area before (0 s) and after photobleaching in a representative experiment using mito-DK (1.9 s, n = 16 cells) or PHB2–DK (43 s, n = 20 cells), respectively. f, Recovery curves of PHB1–DK and PHB2-DK compared with mito-DK (inset). Scale bars, 10 µm (a and b), 200 nm (c) and 2 µm (e). Data are presented as mean values ± s.d.
