Extended Data Fig. 5: Co-immunoprecipitation of prohibitins. | Nature Cell Biology

Extended Data Fig. 5: Co-immunoprecipitation of prohibitins.

From: In situ architecture of the human prohibitin complex

Extended Data Fig. 5

Co-immunoprecipitation of prohibitins from isolated mitochondria. Mitochondrial lysates from PHB-DK1 or PHB-DK2 knock-in cells were immunoprecipitated using Dreiklang as the bait protein. (a) Antibody detection; upper blot: PHB1, lower blot: PHB2. (b) Antibody detection; upper blot: ATP5A, lower blot: COX2. FT1: flow through of wildtype CoIP, FT2: flow through of PHB1-DK CoIP, FT3: flow through of PHB2-DK CoIP. 10 µg of sample was loaded for all samples of Input and flow through, respectively. Source blots are available.

Source data

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