Extended Data Fig. 10: Crosslinking and mass spectrometry analysis of the prohibitin complex.
(a) 1–3: Crosslinking mass spectrometry analyses of the mouse and human prohibitin heteromeric complex. Schematic representation of the distribution of the intra-subunit crosslinks (purple), inter-subunit crosslinks (green), and self-crosslinks (orange) identified in three separate studies (1: Ryl et al., 2020, 2: Schweppe et al., 2017, and 3: Liu et al., 2018). The predicted prohibitin coiled coils are shown in green (left). Crosslinks identified were mapped to the PHB1(5)PHB2(6) heteromeric structure(right). (b) 1–3: Crosslinked sites mapped to the prohibitin heteromeric structures. Crosslinks identified in three separate studies (1: Ryl et al., 2020, 2: Schweppe et al., 2017, and 3: Liu et al., 2018) were mapped to the PHB1(6)PHB2(5) (top) or PHB1(5)PHB2(6) (bottom) heteromeric structures. Crosslinks that validate the structure (<30 Å) are shown in blue while unsatisfied crosslinks (>30 Å) are shown in red.
