Extended Data Fig. 3: Genotyping and expression levels in PHB1-DK and PHB2-DK knock-in clones.
(a, b) PCR analysis of 24 PHB1-DK clones (a) and 28 PHB2-DK clones (b) obtained after single cell selection using FACS and clonal expansion. CTL, parental, non-edited control cell line. (c, d) Protein expression level analysis in five PHB1-DK (c) and five PHB2-DK clones (d). Whole cell extracts of clones were analysed via immunoblotting using antibodies against PHB1 (c) or PHB2 (d), respectively. Wildtype extract (CTL) was loaded as a reference and actin was detected as internal loading control. Band intensities were quantified, PHB1 (c) or PHB2 (d) expression levels corrected for variations in loaded amounts and normalized to the PHB1 (c) or PHB2 (d) expression level in wildtype (CTL) cells. Clone numbers highlighted in red indicate the respective clone chosen for subsequent analysis. Source blots are available.
