Extended Data Fig. 7: Blebbisomes contain RAB proteins and EV marker proteins.
From: Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties
a, TEM micrograph of purified MDA-MB-231 blebbisome displaying a multivesicular endosome. The MVE are pseudo-colored magenta. Box represents enlarged inset of a single MVE displaying multiple intraluminal vesicles. b, Immunoblot analysis of select proteins in MDA-MB-231 and B16-F1 cells, purified blebbisomes (bleb), large EVs (lEV) and small EVs (sEV). c, Immunoblot analysis of annexin A2-positive microvesicles secreted from MDA-MB-231 blebbisomes. n = 3 and data are displayed as mean ± s.e.m. Images represents three independent experiments. d, Replicative immunoblot experiments of EVs secreted from purified MDA-MB-231 blebbisomes. Related to Fig. 5d. Con, control media; EV, secreted EVs from blebbisomes. Source numerical data and unprocessed blots are available in source data.
