Fig. 5: Blebbisomes take up extracellular vesicles.
From: Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties
a, iSIM images of the uptake of EVs labelled with Alexa-647 in actin-stained blebbisomes released by B16-F1, DKO-1 and MDA-MB-231 cells or in prepurified samples of MDA-MB-231 blebbisomes. In the maximum projections, there are horizontal and vertical yellow lines that denote the XZ and YZ orthogonal view. The images are representative of three independent experiments. b, A quantification of the percentage of blebbisomes that contained EV-Alexa-647 signal. The per cent of blebbisomes containing EVs: 53 ± 9% s.e.m., 65 ± 8% s.e.m., 82 ± 11% s.e.m., 43 ± 5% s.e.m. in B16-F1, DKO-1, MDA-MB-231 and prepurified MDA-MB-231 blebbisomes, respectively. The data are from three independent experiments. c, A quantification of the number of EV-Alexa-647 puncta per blebbisome. The number of puncta per blebbisome: 1.31 ± 0.06 s.e.m., 1.61 ± 0.39 s.e.m., 2.75 ± 1.22 s.e.m., 1.19 ± 0.36 s.e.m. in B16-F1, DKO-1, MDA-MB-231 and prepurified MDA-MB-231 blebbisomes, respectively. The data are from three independent experiments. Bleb, purified MDA-MB-231 blebbisomes.
