Fig. 6: Blebbisomes secrete exosomes and microvesicles.
From: Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties
a, A TEM micrograph of purified MDA-MB-231 blebbisome displaying MVE. The MVEs are pseudo-coloured magenta. Inset: enlarged box of a single MVE displaying multiple ILVs. b, Immunofluorescence imaging by iSIM of CD63 and actin in a MDA-MB- 231 blebbisome. c, Immunoblot analysis of control media (Con) and EVs secreted from purified MDA-MB-231 blebbisomes for select EV marker proteins. The images are representative of three independent experiments. d, A quantification of relative signal intensity from c. Each data point represents one independent experiment (see Extended Data Fig. 7d for replicate immunoblots) (n = 3 and data are displayed as mean ± s.e.m.).
