Fig. 7: Blebbisome-like EVs are present in vivo and contain immune checkpoint ligands.
From: Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties
a, A timelapse axially swept light sheet microscopy montage showing blebbisome (bleb) formation from MV3 cells embedded in collagen. Boxes: areas of bleb formation and release. Middle: the timelapse is for the enlarged area in the yellow box. b, A timelapse spinning-disk confocal montage of a bleb released from B16-F1 cell implanted in a zebrafish embryo and stained for actin (Lifeact) and Histone H2B. Box: a blebbisome displaying blebbing behavior. c, A DIC microscopy montage showing bleb in bone marrow. RBC, red blood cell (n = 3 independent isolations). d, An immunoblot analysis of MDA-MB-231 cells, blebs, lEV and sEV for inhibitory immune checkpoint proteins. e, A quantification of relative signal intensity from d. Each data point represents one independent experiment (see Extended Data Fig. 5c for replicate immunoblots) (n = 3, and data are displayed as mean ± s.e.m).
