Fig. 2: Human tNeurons carry proteomic signatures of ageing and AD.
From: Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer’s disease
a, Differential expression of proteins detected in tNeurons of healthy young (n = 3) and aged (n = 3) individuals as well as patients with aged/sAD (n = 6) on PID 40. The top pathways for ageing and sAD proteomes were analysed using gene ontology databases. Comparisons between tNeurons from aged and young donors (left) as well as aged/sAD and aged donors (right). The level of enrichment of the identified proteins (log2-transformed fold change) is represented by coloured circles (increase in red and decrease in blue). b, List of differentially expressed proteins associated with risk genes for age-related neurodegenerative diseases. PD, Parkinson’s disease; ALS/FTD, amyotrophic lateral sclerosis/frontotemporal dementia. c, Cluster heatmap of the Pearson’s correlation coefficients of total tNeuron protein expression (top left). Clusters A to M (right and bottom) show distinct protein expression patterns and the associated gene ontology terms between the young, age and aged/sAD samples. Each line represents the expression of individual protein defined by the relative protein abundance (z-score) across different groups. The white circles represent the average z-score for each cluster and the dashed lines represent the s.d.
