Extended Data Fig. 3: Validation of the quantitative data obtained from the time-lapse airyscan acquisition of Fig. 2.
From: ER-to-Golgi trafficking through a dynamic intermediate cis-Golgi tubular network in Arabidopsis
(a, e, i) Time-lapse airyscan acquisition in root epidermal cells of either mCherry-MEMB12 x NAG1-EGFP (a), mCherry-SYP32 x NAG1-EGFP (e) or mRFP-SYP31 x NAG1-EGFP (i). The time series show an independent MEMB12-, SYP32- or SYP31-compartment that undergo an association with the medial-Golgi. The white star in a indicates a tubular structure. (b, f, j) Upper edge control: individual MEMB12- (b), SYP32- (f) or SYP31- (j) compartments could be tracked for an average track time of 22 sec for MEMB12 and SYP32 and 18 sec for SYP31. (c, d, g, h, k, l) Lower edge controls: one channel was flipped either horizontally or vertically, or rotated by 180°. Whether for MEMB12 (c), SYP32 (g) or SYP31 (k) the number of Golgi-associated compartments drastically decreased when one channel is rotated or flipped while the number of Golgi-independent compartments is strongly increased. For all markers tested, the time of association between either MEMB12 (d), SYP32 (h) or SYP31 (l) with the medial-Golgi strongly decreases when one channel is rotated or flipped. Data are presented as scatter plots with median represented as a line, n = 40 cells for all set of data. In c, d, g, h, k, l, ****P values were all <0.0001. All statistical tests were performed by non-parametric Mann-Whitney two-sided test. All scale bars are 1 µm. Source numerical data are available in source data.
