Extended Data Fig. 4: Validation of the quantitative data obtained from the time-lapse airyscan acquisition of Fig. 3.
From: ER-to-Golgi trafficking through a dynamic intermediate cis-Golgi tubular network in Arabidopsis
(a) Upper edge control: individual MEMB12-compartments could be tracked for an average track time of 15-17 sec. (b, c) Lower edge controls: one channel was flipped either horizontally or vertically, or rotated by 180°. (b) The number of SYP31-associated MEMB12-compartments drastically decreased when one channel is rotated or flipped while the number of SYP31-independent MEMB12-compartments is strongly increased. (c) The time of association between MEMB12 and SYP31 strongly decreases when one channel is rotated or flipped. Data are presented as scatter plots with median represented as a line, n = 40 cells for all set of data. In b, c, ****P < 0.0001. All statistical tests were performed by non-parametric Mann-Whitney two-sided test. Source numerical data are available in source data.
