Extended Data Fig. 2: HSC-derived myeloid cells infiltrate the myocardium post-MI.
a, Heatmap showing Fgd5 and selected cardiac marker gene expression in all cell types from adult mouse heart, obtained from Fei et al., 2022. Expression data is presented as log2-transformed cluster mean transcripts per million (TPM + 1). b, t-SNE plot depicting Fgd5 gene expression in scRNA-seq of mouse BM Linneg c-Kitpos compartment from Dahlin et al., 2018. Expression levels are represented as log10(normalized counts + 1). c, Schematic design of the standard equilibrium tracing using Fgd5CreERT2 mice. Tracing is induced with tamoxifen injections, followed by a 4-week recovery before LAD ligation or sham surgery. Analysis is performed on day 3 post-MI. d,e, Flow cytometry-based plots, illustrating the percentage of lineage-traced dTomatopos cells upon MI or sham surgery. d, Frequencies of dTomatopos lymphoid cells in the BM. e, Frequencies of dTomatopos myeloid cardiac cells are normalized to dTomato label within the corresponding HSC compartment of each mouse. Data is presented as mean with standard deviation. Neutro, Neutrophils; Mono, Monocytes; Macro, Macrophages. Ordinary two-way ANOVA. n = 6 for MI, sham condition. n = 3 for baseline condition. f, Stainings of cardiac sections, illustrating the infiltrating dTomatopos cells upon MI or sham surgery in myocardium areas. Ordinary two-way ANOVA. n = 7-14. IZ, Infarct zone; BZ, Border zone; RZ, Remote zone. For (d-f) cells were isolated in the acute phase at day three after MI. Data are presented as mean ± standard deviation. n indicates the number of biological replicates per condition. For (c), three or more independent experiments were performed.
