Fig. 5: pNSCs are distinct from NCSCs and do not originate from NCCs.

a, Immunofluorescence of brain NSCs, lung pNSCs, tail pNSCs and DRG NCSCs. Data represent three biological replicates. Scale bar, 50 μm. b, Immunofluorescence of differentiated neurons (Tuj1), astrocytes (GFAP) and oligodendrocytes (MBP) from brain NSCs, lung pNSCs, tail pNSCs and DRG NCSCs. Data represent three biological replicates. Scale bar, 50 μm. c, Genetic tracing of NCCs and their progenies. d,e, Schematic for deriving postnatal tail (d) and DRG pNSCs (e) and morphology of primary pNSC clusters. f, Heatmap of global gene expression pattern across samples. n = 3 biological replicates; all replicates are shown. g, Hierarchical clustering of the cell lines based on f.