Fig. 8: Differentiation of pNSCs in situ during early development. | Nature Cell Biology

Fig. 8: Differentiation of pNSCs in situ during early development.

From: Multipotent neural stem cells originating from neuroepithelium exist outside the mouse central nervous system

Fig. 8

a, Genetic tracing of NECs and their progenies. b, Immunohistochemistry of E8.5 Sox1-Cre-mTmG mouse embryos. White arrows indicate migrated GFP+ cells. Data represent three biological replicates. Scale bar, 50 μm. c,d, FACS strategy for deriving lung (c) and tail pNSCs (d) from postnatal Sox1-Cre-mTmG mouse, and morphologies of primary lung (c) and tail pNSC cluster (d) and GFP− cells after culture. e, Schematic of pNSCs’ origination, migration and distribution. f, Strategy of tamoxifen injection to label NECs and morphology of a primary tail pNSC cluster. Scale bar, 50 μm. g, Strategy of tamoxifen injection to label pNSC progenies. h, Immunohistochemistry of DRG and intestine tissues across different time points. White arrows represent GFP+ mature neuronal cells. Data represent three biological replicates. Scale bar, 50 μm. Syn1, Synapsin 1.

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