Fig. 3: Signalling pathways related to guinea pig preimplantation embryo development.
From: The guinea pig serves as an alternative model to study human preimplantation development
a,b, Volcano plot showing the fold change in expression and Bonferroni-adjusted P values (two-sided) of DEGs calculated by Wilcoxon test when comparing EPI and TE (a) and EPI and PE (b). Dot plots of KEGG terms with results from GSEA are also shown, with P values (one-sided, permutation test) less than 0.05 indicated. Dot size indicates the absolute values of normalized enrichment score (NES), colour represents the P value, and the x axis shows the ratio of DEGs corresponding to each KEGG term. c, Schematic of the TRULI treatment protocol. d, Representative immunofluorescence image of SOX2 (yellow), aYAP (cyan), GATA3 (grey) and Hoechst (blue) in embryos treated with control (DMSO) and LATS inhibitor (7.5 µM TRULI). e, Scatter plots showing the total numbers of cells per embryo (Hoechst) and the numbers of cells per embryo for the indicated markers in control (n = 8) and TRULI-treated embryos (n = 9), P values are stated in each figure (two-tailed Mann–Whitney test). Scale bars, 20 µm. f, Schematics of the evolutionarily conserved function of the Hippo signalling pathway in TE formation of mouse human and guinea pig embryos. Notably, in contrast to the mouse, in the human and guinea pig embryo, inhibition of LATS1/2 does not abolish SOX2 expression in inner cells. Schematic created with BioRender.com. Panel f adapted from ref. 3 under a Creative Commons license CC BY 4.0.
